Warning: Table './phcogfir_phcogfs/dr_watchdog' is marked as crashed and should be repaired query: INSERT INTO dr_watchdog (uid, type, message, variables, severity, link, location, referer, hostname, timestamp) VALUES (0, 'php', '%message in %file on line %line.', 'a:4:{s:6:\"%error\";s:14:\"strict warning\";s:8:\"%message\";s:62:\"Non-static method view::load() should not be called statically\";s:5:\"%file\";s:63:\"/home/phcogfir/public_html/sites/all/modules/views/views.module\";s:5:\"%line\";i:1118;}', 3, '', 'https://www.phcogfirst.com/article/822', '', '3.238.173.209', 1620586121) in /home/phcogfir/public_html/includes/database.mysql.inc on line 135

Warning: Table './phcogfir_phcogfs/dr_watchdog' is marked as crashed and should be repaired query: INSERT INTO dr_watchdog (uid, type, message, variables, severity, link, location, referer, hostname, timestamp) VALUES (0, 'php', '%message in %file on line %line.', 'a:4:{s:6:\"%error\";s:14:\"strict warning\";s:8:\"%message\";s:109:\"Declaration of views_handler_field::query() should be compatible with views_handler::query($group_by = false)\";s:5:\"%file\";s:83:\"/home/phcogfir/public_html/sites/all/modules/views/handlers/views_handler_field.inc\";s:5:\"%line\";i:0;}', 3, '', 'https://www.phcogfirst.com/article/822', '', '3.238.173.209', 1620586121) in /home/phcogfir/public_html/includes/database.mysql.inc on line 135

Warning: Table './phcogfir_phcogfs/dr_watchdog' is marked as crashed and should be repaired query: INSERT INTO dr_watchdog (uid, type, message, variables, severity, link, location, referer, hostname, timestamp) VALUES (0, 'php', '%message in %file on line %line.', 'a:4:{s:6:\"%error\";s:14:\"strict warning\";s:8:\"%message\";s:136:\"Declaration of views_handler_argument::options_validate() should be compatible with views_handler::options_validate($form, &$form_state)\";s:5:\"%file\";s:86:\"/home/phcogfir/public_html/sites/all/modules/views/handlers/views_handler_argument.inc\";s:5:\"%line\";i:0;}', 3, '', 'https://www.phcogfirst.com/article/822', '', '3.238.173.209', 1620586121) in /home/phcogfir/public_html/includes/database.mysql.inc on line 135

Warning: Table './phcogfir_phcogfs/dr_watchdog' is marked as crashed and should be repaired query: INSERT INTO dr_watchdog (uid, type, message, variables, severity, link, location, referer, hostname, timestamp) VALUES (0, 'php', '%message in %file on line %line.', 'a:4:{s:6:\"%error\";s:14:\"strict warning\";s:8:\"%message\";s:112:\"Declaration of views_handler_argument::query() should be compatible with views_handler::query($group_by = false)\";s:5:\"%file\";s:86:\"/home/phcogfir/public_html/sites/all/modules/views/handlers/views_handler_argument.inc\";s:5:\"%line\";i:0;}', 3, '', 'https://www.phcogfirst.com/article/822', '', '3.238.173.209', 1620586121) in /home/phcogfir/public_html/includes/database.mysql.inc on line 135

Warning: Table './phcogfir_phcogfs/dr_watchdog' is marked as crashed and should be repaired query: INSERT INTO dr_watchdog (uid, type, message, variables, severity, link, location, referer, hostname, timestamp) VALUES (0, 'php', '%message in %file on line %line.', 'a:4:{s:6:\"%error\";s:14:\"strict warning\";s:8:\"%message\";s:134:\"Declaration of views_handler_filter::options_validate() should be compatible with views_handler::options_validate($form, &$form_state)\";s:5:\"%file\";s:84:\"/home/phcogfir/public_html/sites/all/modules/views/handlers/views_handler_filter.inc\";s:5:\"%line\";i:0;}', 3, '', 'https://www.phcogfirst.com/article/822', '', '3.238.173.209', 1620586121) in /home/phcogfir/public_html/includes/database.mysql.inc on line 135

Warning: Table './phcogfir_phcogfs/dr_watchdog' is marked as crashed and should be repaired query: INSERT INTO dr_watchdog (uid, type, message, variables, severity, link, location, referer, hostname, timestamp) VALUES (0, 'php', '%message in %file on line %line.', 'a:4:{s:6:\"%error\";s:14:\"strict warning\";s:8:\"%message\";s:110:\"Declaration of views_handler_filter::query() should be compatible with views_handler::query($group_by = false)\";s:5:\"%file\";s:84:\"/home/phcogfir/public_html/sites/all/modules/views/handlers/views_handler_filter.inc\";s:5:\"%line\";i:0;}', 3, '', 'https://www.phcogfirst.com/article/822', '', '3.238.173.209', 1620586121) in /home/phcogfir/public_html/includes/database.mysql.inc on line 135

Warning: Table './phcogfir_phcogfs/dr_watchdog' is marked as crashed and should be repaired query: INSERT INTO dr_watchdog (uid, type, message, variables, severity, link, location, referer, hostname, timestamp) VALUES (0, 'php', '%message in %file on line %line.', 'a:4:{s:6:\"%error\";s:14:\"strict warning\";s:8:\"%message\";s:127:\"Declaration of views_plugin_query::options_submit() should be compatible with views_plugin::options_submit($form, &$form_state)\";s:5:\"%file\";s:81:\"/home/phcogfir/public_html/sites/all/modules/views/plugins/views_plugin_query.inc\";s:5:\"%line\";i:0;}', 3, '', 'https://www.phcogfirst.com/article/822', '', '3.238.173.209', 1620586121) in /home/phcogfir/public_html/includes/database.mysql.inc on line 135

Warning: Table './phcogfir_phcogfs/dr_watchdog' is marked as crashed and should be repaired query: INSERT INTO dr_watchdog (uid, type, message, variables, severity, link, location, referer, hostname, timestamp) VALUES (0, 'php', '%message in %file on line %line.', 'a:4:{s:6:\"%error\";s:14:\"strict warning\";s:8:\"%message\";s:139:\"Declaration of views_plugin_argument_validate::options_submit() should be compatible with views_plugin::options_submit($form, &$form_state)\";s:5:\"%file\";s:93:\"/home/phcogfir/public_html/sites/all/modules/views/plugins/views_plugin_argument_validate.inc\";s:5:\"%line\";i:0;}', 3, '', 'https://www.phcogfirst.com/article/822', '', '3.238.173.209', 1620586121) in /home/phcogfir/public_html/includes/database.mysql.inc on line 135

Warning: Table './phcogfir_phcogfs/dr_watchdog' is marked as crashed and should be repaired query: INSERT INTO dr_watchdog (uid, type, message, variables, severity, link, location, referer, hostname, timestamp) VALUES (0, 'php', '%message in %file on line %line.', 'a:4:{s:6:\"%error\";s:14:\"strict warning\";s:8:\"%message\";s:130:\"Declaration of views_plugin_row::options_validate() should be compatible with views_plugin::options_validate(&$form, &$form_state)\";s:5:\"%file\";s:79:\"/home/phcogfir/public_html/sites/all/modules/views/plugins/views_plugin_row.inc\";s:5:\"%line\";i:0;}', 3, '', 'https://www.phcogfirst.com/article/822', '', '3.238.173.209', 1620586121) in /home/phcogfir/public_html/includes/database.mysql.inc on line 135
Evaluation of the impact of regional variation on b-sitosterol content in Asteracantha longifolia Nees. seeds using HPTLC and HPLC technique
Home | Articles
Published on:21 Aug 2013
Pharmacognosy Communications, 2013; 3(3):16-21
Research Article | doi:10.5530/pc.2013.3.4

Evaluation of the impact of regional variation on b-sitosterol content in Asteracantha longifolia Nees. seeds using HPTLC and HPLC technique


Authors and affiliation (s):

Sunita Shailajan*, Neelam Sayed and Bhavesh Tiwari

Herbal Research Lab, Ramnarain Ruia College, Matunga (E), Mumbai 400019, India

Abstract:

Background: Seeds of Asteracantha longifolia Nees. (Acanthaceae) are an important ingredient in many traditional/herbal formulations used in the management of a wide range of reproductive disorders. They are reported to posses various therapeutically active phytochemical constituents like asteracanthine, lupeol, asterol, β-sitosterol etc. Materials and Methods: In this research work, seeds collected from various geographical regions were analyzed using two chromatographic techniques (HPTLC and HPLC). A comparative evaluation of β-sitosterol content in respective samples using these techniques has been carried out and discussed. Both the chromatographic methods have been validated as per ICH guidelines and applied to evaluate the content of β-sitosterol in formulations containing seeds of A. longifolia. Results: Variation in the β-sitosterol content of A. longifolia seeds collected from different geographical regions was observed. Seeds collected from Aligarh were found rich in β-sitosterol while sample from Pen showed minimum content. The methods were also found applicable to evaluate the β-sitosterol content in some traditional/ herbal formulations of A. longifolia seeds. Conclusions: Impact of geographical variation on the content of β-sitosterol in the plant samples was clearly evident from the findings of the current work. There exists a definite impact of climatic and edaphic conditions over the marker content of medicinal plants.

KE YWORDS: Asteracantha longifolia Nees., HPLC, HPTLC, regional variation, seeds.

 

Articles in PDF, ePUB and Full text are attached to this page. In order to download, print or access these formats you must be logged in.




 

Article Links