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Published on:11/2010
Pharmacognosy Journal, 2010; 2(16):05 -11
Research Article | doi:10.1016/S0975-3575(10)80043-4

Safety Assessment of Centella asiatica in albino rats


Authors and affiliation (s):

Madhuri Oruganti1*, Birendra Kumar Roy2, Kaushal Kumar Singh3, Raju Prasad2, Subodh Kumar4

1Central Council For Research In Ayurveda & Siddha, Dept. of AYUSH, Ministry of Health & Family Welfare, Janakpuri, New Delhi-58.

2Deptt. of Pharmacology and Toxicology, College of Veterinary Science and Animal Husbandry, Ranchi-834006, Jharkhand, India.

3Deptt. of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Ranchi-834006, Jharkhand, India.

4Molecular Genetic Lab, Dept of Genetics, College of Veterinary Science and Animal Husbandry, Ranchi-834006, Jharkhand, India.

Abstract:

Aim: Present study was designed to assess the safety levels of Centella asiatica (aerial parts) after 30 days oral administration in albino rats. Materials & Methods: Control group (I) received distilled water and test groups (II, III and IV) received graded dosage of 250, 500, 1,000 mg/kg. b.wt orally respectively for a period of 30 days. Changes in body weight were recorded at 10 days interval. On 32nd day blood samples were collected and the whole blood was used for hematological studies, DNA fragmentation assay and marker enyme levels were assessed in serum. The vital tissues (Liver, Kidney, Heart, Spleen, and Brain) were dissected out and utilized for viability assay (Trypan blue dye exclusion test), evaluation of apoptosis (fluorescence microscopy) and histopathological studies. Results: Group III and IV animals showed a significant increase in serum biomarkers (AST, ALT, BUN, Creatinine) and apoptotic index. There was statistically significant decrease in viability count in treatment groups in comparison to the control group. Histopathology also revealed a significant hepatic damage and a moderate degree of changes in the renal tissue. Conclusion: Based on the above observations it was concluded that the administration of Centella asiatica at 1,000 mg/kg b.wt for a period of 30 days may cause a significant damage to liver tissue in rats. Significance & Impact of the study: This study signifies the organ specific toxicity of Centella asiatica.

Key words: Mandookaparni, Toxicity, Apoptosis, Herbal Medicine.

 

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