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Published on:23rd Nov, 2012
Pharmacognosy Journal, 2012; 4(32):38-44
Research Article | doi:10.5530/pj.2012.32.8

In vivo and in vitro anti-inflammatory activity of Harrisonia perforata root extract


Authors and affiliation (s):

*Pattama Somsila, Nijsiri Ruangrungsib, Wacharee Limpanasitikula and Chandhanee Itthipanichponga

aDepartment of Pharmacology, Faculty of Medicine

bDepartment of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand

Abstract:

Background: Harrisonia perforata root is one of the five medicinal plants in Bencha-Loga-Wichien remedy used for the treatment of fever in Thai traditional medicine. The aim of this study was to investigate the anti-inflammatory effect and the action mechanism of the ethnolic extract from the root of H. perforata in vivo and in vitro. Methods: Male Wistar rats were divided into 6 groups and treated with either the ethanolic extract of H. perforata root at dose 5–400 mg/kg or indomethacin 5 mg/kg or vehicle. Rat paw volume was measured at 1, 2, 3, 4, 5 and 6 h after carrageenan injection. The in vitro mechanism of the antiinfammatory response was investigated through the measurement of mRNA expression of proinflammatory cytokines, TNF-α, IL-6 and IL-1β. Lipopolysaccharidestimulated macrophage, J774A.1 cell, were exposed to different concentration of H. perforata root extract (12.5–50 μg/ml). mRNA expression, TNF-α, IL-1β and IL-6, were determined by real-time RT-PCR. Cell viability was measured by rezasurin assay. Results: H. perforata root extract 50–400 mg/kg. significantly reduced the effect of acute inflammation in rat paw edema by 28.49%- 65.05% at 2 hour after carrageenan injection. Cell viability were not significantly altered by the presence of H. perforata extract 3.125–50 μg/ml. The maximum inhibitory effect for TNF-α, IL-1β and IL-6 were 49.83, 47.27 and 32.16% respectively. The concentration of the extract produced maximum effect were 50 μg/ml for both TNF-α and IL-1β but for IL-6, it was 12.5 μg/ml. Conclusion: The ethanolic extract from H. perforata root possesses anti-inflammatory effect. This may occur through suppression of proinflammatory cytokines.

Keywords: Harrisonia perforata, antiinflammation, proinflammatory cytokines.

 

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