Home | Articles
Published on:26th May 2014
Pharmacognosy Journal, 2014; 6(4):59-67
Research Article | doi:10.5530/pj.2014.4.9

Protective effect of cissampelos pareira linn. Extract on gentamicin-induced nephrotoxicity and oxidative damage in rats

Authors and affiliation (s):

D. R Ch Sekhara Reddy1*, G. Siva Kumar 2, B. M. Vrushabendra Swamy2 and K. Phani Kumar1

1Acharya Nagarjuna University College of Pharmaceutical Sciences, Nagarjuna Nagar, Guntur-522510, India

2Gautham College of Pharmacy, Bhuvaneswari Nagar, Bengaluru-560032, India.


Objective: To evaluate the potential nephroprotective and antioxidant activity of hydroalcoholic Cissampelos pareira (C. pareira) whole plant extract using gentamicin-induced rats. Methods: For studying acute toxicity study, single oral dose of 2g/kg hydroalcoholic extract of C. pareira was evaluated in rats by oral gavage. The nephroprotective activity was evaluated using gentamicin-induced model in rats. In-vitro antioxidant activity was evaluated by using DPPH assay and reducing power assay. In-vivo antioxidant activity was evaluated by using glutathione and lipid peroxidation estimations in gentamicin-induced rats. Hydroalcoholic C. pareira whole plant extract was given at a dose of 200 and 400 mg/kg p.o. Results: For acute toxicity testing rats administered with the extract at a dose 2 g/ kg. the result showed no toxicity. Hydroalcoholic C. Pareira whole plant extract (200 and 400 mg/kg p.o) significantly decrease the elevated urinary glucose levels in the urine, decrease the elevated urea and creatinine levels in blood and increase the urinary creatinine levels in gentamicin-induced nephrotoxic rats. The extract had shown significant dose dependent increase in the DPPH and reducing power activity. There were a dose dependent decreasing and increasing of lipid peroxidation, glutathione levels in hydoalcoholic extract treated groups respectively. Conclusion: This study exhibits that hydroalcoholic C. pareira whole plant extract poses nephroprotective activity which may be due to its antioxidant activity.

Key words: Gentamicin, Cissampelos pareira, creatinine, urea, glucose, lipid peroxidation, glutathione, reducing power, DPPH.


Articles in PDF, ePUB and Full text are attached to this page. In order to download, print or access these formats you must be logged in.
This question is for testing whether you are a human visitor and to prevent automated spam submissions.
16 + 3 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.


Article Links