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Phytochemical Screening and In vitro Anti-inflammatory Activity of the Stem of Coleus forskohlii.
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Published on:7, 2011
Pharmacognosy Journal, 2011; 3(23):75-79
Original Article | doi:10.5530/pj.2011.23.11

Phytochemical Screening and In vitro Anti-inflammatory Activity of the Stem of Coleus forskohlii.


Authors and affiliation (s):

Darsan B Menon1 and K. Latha*2

1Research Scholar, Dept. of Biotechnology, Karpagam University, Coimbatore, India – 641021.

2Project Coordinator, Phytopharma lab, Herbal Division, T.Stanes and Company Ltd., Coimbatore, India – 641018.

Abstract:

The extracts of Coleus forskohlii prepared by using hexane, chloroform, methanol, 80% methanol and water as solvents were screened for secondary metabolites and for its invitro anti-inflammatory activity. The extracts revealed the presence of alkaloids, phenols, tannins, proteins, carbohydrates, saponins, glycosides and cardiac glycosides and most of these compounds were observed in the aqueous, 80% methanol and methanolic extracts. DPPH antioxidant assay and the in vitro anti-inflammatory activity assays viz., BSA anti-denaturation and HRBC membrane stabilization assay indicated that the aqueous and methanolic extracts of Coleus forskohlii possess constituents with anti-inflammatory properties. TLC profile of the methanolic extract confirmed the presence of forskolin, which is a major bioactive compound isolated from the roots of Coleus forskohlii. Of all the extracts that were tested for their in vitro anti-inflammatory activity, methanolic and aqueous extracts showed maximum activity.

Key words: Coleus forskohlii, anti-inflammatory, DPPH and TLC.

 

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